Skip to main content
Fig. 2 | Molecular Brain

Fig. 2

From: Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Fig. 2

LncC11orf54-1 is downregulated in hBMECs with meningitic E. coli infection. A Expression of lncC11orf54-1, mgU2-19, and mgU2-30 in hBMECs infected with E. coli for 0 h (control, lane 1), 1 h (lane 2), 2 h (lane 3) or 3 h (lane 4) using qPCR analysis. GAPDH was used as the internal reference for the qPCR. Data are presented as mean ± SD from three independent experiments. **p < 0.01, and ***p < 0.001 by student's t-test. B Visualization of lncC11orf54-1 in the ZENBU browser, showing exonic expression signaling in several different visualizations. C Subcellular localization of lncC11orf54-1 (red) in hBMECs by FISH assay. 4′,6-diamidino-2-phenylindole (DAPI) staining is shown in blue. Scale bars, 20 μm. D Nuclear/cytoplasmic localization analyses of lncC11orf54-1 in hBMECs by qPCR. The 18S and U6 distribution were selected as the cytoplasmic and nuclear control, respectively. E Expression of lncC11orf54-1 and mgU2-30 in hBMECs infected with E. coli for 0 h (control, lane 1), 1 h (lane 2), 2 h (lane 3) or 3 h (lane 4) using Northern blotting

Back to article page

Molecular Brain

ISSN: 1756-6606