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Fig. 3 | Molecular Brain

Fig. 3

From: Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Fig. 3

Meningitic E. coli infection induces the translocation of coilin. A hBMECs were infected with or without meningitic E. coli, and the translocation of coilin (red) was detected by fluorescence microscopy. DAPI staining was shown in blue. Scale bars, 50 μm. B Nuclear-to-cytoplasmic redistribution analyses of coilin in E. coli-stimulated hBMECs. Western blotting analyses of coilin were performed on whole cell lysate, nuclear, and cytoplasmic fractions. GAPDH was used as the loading control for whole-cell lysate and cytoplasmic fractions, whereas for the nucleus fraction, lamin B was used as the loading control. C RNA FISH analyses and immunofluorescent analyses determining the co-localization of lncC11orf54-1 (red) and coilin (green) in hBMECs with or without E. coli challenge. DAPI staining was shown in blue. Scale bars, 50 μm

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Molecular Brain

ISSN: 1756-6606