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Fig. 1 | Molecular Brain

Fig. 1

From: Cell-type-specific DNA methylation analysis of the frontal cortices of mutant Polg1 transgenic mice with neuronal accumulation of deleted mitochondrial DNA

Fig. 1

Epigenetic characterization of the brains of mutant Polg1 Tg mice. a The transgene construct of mutant Polg1 Tg mice. The mutant mice express proofreading-deficit Polg1 due to a D181A mutation under the neuron-specific Camk2a promoter. The upstream region of Camk2a (about 8.8 kb) contains a part of the neighboring gene, Arsi. Black bars indicate hypermethylated DMRs. Hypermethylated region was detected around Arsi in both cell types. Three hypermethylated regions were additionally detected in the Camk2a promoter in nonneurons. b The number of DMRs and their associated genes in neurons and nonneurons. Venn diagrams are drawn based on the DMR-associated genes. c Chromosomal location and top hit significant Gene Ontology terms. See Additional file 3: Table S5 for detailed result of Gene Ontology analysis. d Top hit DMRs in neurons and nonneurons. DMRs on the transgene (i.e., Arsi and Camk2a) or those with no gene symbols were excluded from the list. See Additional file 3: Tables S1–S4. e Comparison of DMR-associated genes in the mutant mice with previously reported DEGs in the mutant mice and DMRs in patients with BD. Arsi Arylsulfatase I, BD bipolar disorder, Camk2a calcium/calmodulin-dependent protein kinase type II subunit alpha, DEG differentially expressed gene; DMR differentially methylated region, Polg1 DNA polymerase subunit gamma 1

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