Fig. 4

Effect of optical suppression of D2-receptor-expressing neurons in the NAc on neuropathic pain. a Schematic showing that AAV-Flex-ArchT-GFP was microinjected into the NAc of D2-cre mice. b Experimental timeline. c Examples of biocytin-stained ArchT-GFP-expressing neurons in a D2R-cre mouse (upper) and representative current-clamp trace from a NAc neuron expressing ArchT showing reliable suppression of the firing of neurons during photostimulation (orange line) (lower). d-f Effects of optical suppression of D2-receptor-expressing neurons in the NAcMed, NAcLat and NAcCo in mice. Latency in the response to thermal stimulation under a neuropathic pain-like state by optical stimulation of the NAcMed (d), NAcLat (e) and NAcCo (f) in Wild-type/ArchT and D2-cre/ArchT mice. Data are presented as the mean ± SEM of 5-6 animals. ^***p < 0.001 vs. Wild-type/ArchT ligation contralateral paw; ^###p < 0.001 vs. D2-cre/ArchT ligation contralateral paw; ^$$$p < 0.001 vs. Wild-type/ArchT ligation ipsilateral paw