Fig. 4

Use of the PARTS method to trace the broad-spectrum upstream input network of the VTA. A Schematic diagram of retrograde tracing by the PARTS method. Helper virus that expresses the TVA receptor fused with nuclear EGFP was intravenously injected into C57BL/6 J adult mice, and RV-EnvA-SAD-B19-ΔG-DsRed was injected into the VTA after 3 weeks. Then, the mice were sacrificed after another week, and the upstream inputs of the VTA were imaged. B Fluorescence expression at the injection site (VTA), scale bar = 200 μm. C Fluorescence expression in some upstream brain regions of the VTA. Red fluorescence signals were colabelled with green fluorescence signals, scale bar = 200 μm. CTX cerebral cortex, LHb lateral habenula, LHA lateral hypothalamic area, ZI zona incerta, MRN midbrain reticular nucleus, CBN cerebellar nuclei