Fig. 6

Use of the cPARTS method to trace the cell-type-specific upstream input network of the VTA. A Schematic diagram of retrograde tracing by the cPARTS method. Cre-dependent helper virus that expresses the TVA receptor fused with nuclear EGFP was intravenously injected into VGAT-Cre adult mice, and RV-EnvA-SAD-B19-ΔG-DsRed was injected into the VTA after 3 weeks. Then, the mice were sacrificed after another week, and the upstream inputs of the VTA were imaged. B Fluorescence expression at the injection site (VTA), scale bar = 200 μm. C Fluorescence expression in some upstream brain regions of the VTA. Red fluorescence signals were colabelled with green fluorescence signals, scale bar = 200 μm. GPe globus pallidus, external segment; LHA lateral hypothalamic area; ZI zona incerta; PRNr pontine reticular nucleus; LDTg laterodorsal tegmental nucleus; VNC vestibular nuclei