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Table 1 Comparison of viral tracers and methods of retrograde tracing

From: Brain-wide TVA compensation allows rabies virus to retrograde target cell-type-specific projection neurons

Method/virus

Efficiency

Preparation

Toxicity

Brain region selectivity

Cell-type-specific

AAV2-retro

High

Easy

Low

Yes

Yes

AAV & AAVR

Medium

Easy

Low

No

Yes

CAV2

Low

Easy

Low

Yes

Yes

CAV2 & CAR

Medium

Easy

Low

No

Yes

RV-ΔG(N2cG)

High

Easy

High

No

No

PARTS

High

Easy

High

No

No

cPARTS

High

Easy

High

No

Yes

  1. Efficiency and brain region selectivity of viral tracers are relative to other viruses, and in most cases, researchers can choose the appropriate viral tracers according to their different experimental needs