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Fig. 5 | Molecular Brain

Fig. 5

From: Tumor suppression and improvement in immune systems by specific activation of dopamine D1-receptor-expressing neurons in the nucleus accumbens

Fig. 5

Effect of pharmacogenetic activation of D1-receptor-expressing neurons in the medial shell of the nucleus accumbens on the state of exhaustion of CD8+ T cells. a, b Effects of activation of D1-receptor-expressing neurons by hM3Dq in the medial shell of the nucleus accumbens of D1-cre mice with hM3Dq by repeated administration of CNO on TIM-3+ (a) and TIM-3+ PD-1+ (b) levels of CD8+ T cells in the spleen 15 days after LLC transplantation (Mann–Whitney test, *p < 0.05 vs. D1-WT/hM3Dq). c Relationship between the tumor volume and percentage of TIM-3+ PD-1+ in CD8+ T cells from the spleen of D1-WT and D1-Cre mice with hM3Dq, which had been implanted with LLC, after activation of D1-receptor-expressing neurons in the medial shell of the nucleus accumbens (Pearson's correlation coefficient test, r2 = 0.6030, p = 0.0030). d, e Effects of activation of D1-receptor-expressing neurons in the medial shell of the nucleus accumbens of D1-cre mice with hM3Dq by repeated administration of CNO on LAG-3+ (d) and LAG-3+ PD-1+ (e) levels of CD8+ T cells in the spleen 15 days after LLC transplantation (Mann–Whitney test, *p < 0.05, **p < 0.01 vs. D1-WT/hM3Dq). (f) Relationship between tumor volume and the percentage of LAG-3+ PD-1+ in CD8+ T cells from the spleen of D1-WT and D1-Cre mice with hM3Dq, which had been implanted with LLC, after activation of D1-receptor-expressing neurons in the medial shell of the nucleus accumbens by repeated administration of CNO (Pearson's correlation coefficient test, r2 = 0.6833, p = 0.0009). Each point represents the mean ± S.E.M. of 6 samples

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