Fig. 2

Ectopically localized SorLA cytoplasmic domain recruits PICK1. A COS7 cells were transfected with T70mV-SorLA-CD, T70mV-Sortilin-CD or T70mV-nanoLuc (green) and co-transfected with tdTomato-PICK1, tdTomato-GGA2 or PSD95-tdTomato (magenta) as indicated. Magnifications of selected areas (boxes) are shown as insets. mVenus and tdTomato signals were enhanced by immunostainings with respective antibodies. B COS7 cells were transfected with the mutated SorLA constructs T70mV-SorLA-CD-AAA or T70mV-SorLA-CD-VAA (green) and co-transfected with tdTomato-PICK1 or tdTomato-GGA2 (magenta) as indicated. C Ectopic localization of T70mV-nanoLuc at mitochondria. COS7 cells were transfected with T70mV-nanoLuc (green) and stained with an antibody against the mitochondrial protein Tom20 (magenta). D Summary of the recruitment of the tdTomato fusion constructs of PICK1, GGA2 and PSD95 by the analyzed cytoplasmic domains to mitochondria. Scale bars: 10 µm