Fig. 2

OGA activity is decreased in mice exposed to Thiamet G (TG). A OGA activity assay in cortical homogenates from male and female mice treated with either vehicle (0.9% saline; Sal) or TG. The rate of 4MU release over time was analyzed by two-way ANOVA and sex differences assessed by Student’s t-tests and false discovery rate controlled using the Benjamini–Hochberg method (q* = 0.05). ***p < 0.0001. B Representative image and western blot analysis of O-GlcNAcylated protein levels in saline-treated females (FS), TG-treated females (FT), saline-treated males (MS), and TG-treated males (MT) measured with the CTD110.6 antibody. ***p < 0.001, and ****p < 0.0001 compared to saline controls by student t-test (2-way ANOVA and post-hoc Tukey). C, D Representative image and western blot analyses of OGT and OGA protein levels