Fig. 1
From: Loss of calsyntenin paralogs disrupts interneuron stability and mouse behavior
Generation of calsyntenin (Clstn) triple knockout (TKO) mice. a Representation of calsyntenin (CLSTN) domains and target regions of single-guide RNAs (sgRNAs). Each CLSTN has two cadherin domains (blue box), one LG/LNS domain (red box), and one or two kinesin-binding sites (blue line). The target region is designed to be upstream exon (exon 3 or exon 4) and is indicated by an arrowhead in the figure. b DNA sequences of modified Clstn genes in KO mice. The PAM sequences are highlighted in orange; the sgRNA-targeting sequences in blue; deletion mutations (−) in red. c Western blot of protein lysates of CLSTN-TKO whole brain shows the success of knockout at the protein level