Fig. 4

The tests for evaluating anxiety levels. Anxiety-related behavior of TKO mice. a Time spent in the open arms during the test. TKO mice showed a longer search time in the open arm immediately after the start of the test, but after 5 min, the time spent in the open arm was similar to that of the WT mice. b, c Time spent in the dark chamber for the light–dark preference test. In the light–dark preference test, WT and TKO mice showed similar levels of anxiety. All data are presented as the mean ± standard error of the mean (WT mice, n = 17; TKO mice, n = 16). P values for differences between genotypes were determined by Welch’s t-test (c) or calculation of genotype effect in two-way repeated-measures analysis of variance (a, b)