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Fig. 1 | Molecular Brain

Fig. 1

From: Hif1α-dependent hypoxia signaling contributes to the survival of deep-layer neurons and cortex formation in a mouse model

Fig. 1

Conditional ablation of the Hif1α gene. Expression of Hif1α mRNA was detected using whole-mount and cryosection in situ hybridization of E8.5 (A and B) and E9.5 (C and D) embryos. Neural tube is indicated by the dotted line. hm, head mesenchyme; nt, neural tube; Scale bars, 100 μm. Eight (A and B) and four (C and D) independent experiments are performed and one representative image is shown, respectively. Expression levels of Hif1α and its target genes at E8.5 (E) and E9.5 (F) examined using RT-qPCR. The mRNA levels of target genes were normalized to those of Gapdh, and the relative values are presented as bars. White bar, WT; gray bar, KO. Data are the mean ± S.E.M of five embryos. Statistical differences were assessed using Student’s t-test, * p < 0.05. WT, wild type; KO, knockout

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Molecular Brain

ISSN: 1756-6606