Fig. 5

Predominant apoptotic elimination of Ctip2+ deep-layer neurons affects cortical layer formation. Apoptotic cells and Satb2+ (A, B) or Ctip2+ (C, D) cells in coronal sections of the KO cortex at E18.5 are shown. Higher magnification images of the area enclosed by rectangular dotted line are shown as insets (B, D). Satb2+ cells accumulating at the apical side of the apoptotic cell aggregate are indicated by yellow arrowheads. Ctip2+ cells distributed near the pial surface are indicated by white arrowheads. Scale bars, 100 μm (A–D). Three independent experiments are performed and one representative image is shown, respectively. The percentage of cleaved caspase3 + and Satb2+ or Ctip2+ cells is presented as a bar graph (E). Data are the mean ± S.E.M of six sections. Statistical differences were assessed using Student’s t-test, * p < 0.05