Fig. 1

Characterization of the PV-Cre-Mediated CTCF cKO Mice. A Immunofluorescence analysis of CTCF in the hippocampus of 8-week-old wild-type and CTCF-cKO mice shows depletion of CTCF from parvalbumin inhibitory neurons. B Body weight over time. The CTCF-cKO mice exhibit significantly less weight by 13 weeks after birth. n = 7 for each group. *p = 0.016, **p = 0.005, ***p = 0.0006. Error bars represent SEM. C Survival curves of CTCF-cKO mice. 60% died by the time of 20 weeks after birth (n = 20). D 12 week old CTCF-cKO mice started to show an abnormal limb-clasping reflex when suspended by the tail which gradually got worse. WT = 5 animals, cKO = 12 animals. *p = 0.025, ***p = \({4.71}^{-6},\)***p = \({4.4}^{-16}\). Error bars represent SEM. E Tunel analysis revealed no apoptosis in CTCF cKO mice in the hippocampus-DG, cerebellum, striatum and prelimbic prefrontal cortex of 17 week old mice compared to WT. Positive control was performed with Dnase1 treatment and the image taken on the prelimbic prefrontal cortex. n = 4 for each group