Fig. 2

Adult CTCF-cKO mice demonstrate mild motor problems and reduction in anxiety-related behavior. Adult (8 weeks old) CTCF-cKO (n = 12) and WT (n = 9) performance in locomotor behavioral tests (A-B). A time spent on rotarod and B Distance move total. CTCF-cKO mice spent less time on the rotarod (*p = 0.035) and moved significantly less compared to the control group (***p = 0.00071). C, D Results of the light/dark transition test. CTCF cKO mice spent greater time in light zone (C, ***p = \({6.25}^{-5}\)) however, similar number of entries into light zone (D). E, F Results of the elevated plus maze test. CTCF cKO mice spent less time in close arms (**p = 0.0014) however, more time in the center (**p = 0.0068) (E) and lower number of entries into close arm (**p = 0.0063) (F). G Results of the three-chambered social test. CTCF-cKO mice displayed no preference to spend time in a chamber with a stranger mouse (S) in comparison to empty chambers (E) (*p = 0.019). two-tailed, paired t test. Values in graphs are expressed as the mean ± SEM