Fig. 5

CTCF- cKO mice display decline in PV neurons and altered electrophysiological properties. A, B Results of the single-nuclei RNA-seq (snRNA-seq) from the 2 weeks mice forebrain. Picture on left shows all sequenced nuclei. Picture on right is zoom in on area with majority of parvalbumin-expressing neurons (represented by square on left side of panel). CTCF cKO mice are more densely clustered and less parvalbumin-expressing cells (A). Gene Ontology analysis of differentially expressed genes. Enrichment of upregulated genes highlighted ion channel activity, specifically potassium ion channels and genes that are involved in inhibitory neuron identity (B)