Fig. 1

Tmem108 expression profile in several areas of the brain. A Relative expression of Tmem108 in postnatal mice brain was quantified by qPCR. Gapdh was used as an internal control (Internal control of the below is same in qPCR assay), and Tmem108 expression in P0 mice was defined as 1; Wild-type male mice per group, n = 5. B Representative image of TMEM108 level in postnatal mice brains verified by western blotting. β-ACTIN was used as an internal control (Internal control of the below is the same in western blotting). C Tmem108 relative expression in the different areas of the adult mice brain. Tmem108 expression in CB was defined as 1; Wild-type male mice, n = 3; CB cerebellum, THY thalamus, HP hippocampus, CC corpus callosum, CT cerebral cortex, STR striatum, PFC prefrontal cortex, OB olfactory bulb. D Representative image of TMEM108 level in different areas of adult mice brain verified by western blotting. E Tmem108 relative expression of CC decreased in postnatal mice development. Tmem108 expression in P0 mice CC was defined as 1; Wild-type male mice per group, n = 3 (Unpaired T-test were made to compare with P0 data, n.s., not significant, * p < 0.05, ** p < 0.01). F Tmem108 expression in young mice CC was higher than in adult mice CC by X-gal staining. Tmem108 −/− mice per group, n = 3. G Co-staining indicated Tmem108 expression mostly colocalized with OLs in the corpus callosum of young mice. X-gal staining represented β-gal expression downstream of the Tmem108 promoter, and the arrows showed the X-gal staining dots in Tmem108 mutant mice (P14, Tmem108 −/− mice n = 3, scale bar = 20 μm)