Fig. 2

Hypermyelination of the corpus callosum in Tmem108 mutant mice. A Representative electron microscopy of axons in the CC of young animals at P14. Same littermate male mice were used. B Percentages of CC myelinated axons were quantified in the young mice(P14). C Scatter plot of g-ratio values and g-ratio mean in the CC of the young mice(P14). D Mean g-ratio values of CC myelinated axons with different diameters in the young mice(P14). E Representative electron microscopy of axons in the CC of adult mice (2 M). Same littermate male mice were used. F Percentages of CC myelinated axons were quantified in the adult mice. G Scatter plot of g-ratio values and g-ratio mean in the CC of the adult mice. H Mean g-ratio values of CC myelinated axons with different diameters in the adult mice. (Red scale bar = 2 μm, white scale bar = 200 nm; Male mice per group n = 3, over 200 fibers per each type of animal were analyzed; Values were means ± SEM; Unpaired T-test were made between the groups; n.s., not significant, * p < 0.05, *** p < 0.001). I–K Small-diameter CC axons in Tmem108 mutant mice were readily myelinated. I Percentage of myelinated axons with different diameters in the total myelinated axons. J Diameter of myelinated axons in the adult mice. K Myelin thickness of CC axons in the adult mice (Tmem108 + / + mice n = 3, axon fiber n = 684; Tmem108 −/− mice n = 3, axon fiber n = 757; Unpaired T-test were made between the groups; n.s., not significant)