Fig. 7

Tmem108 mutant mice exhibited mania-like behavior and were easily induced epilepsy. A Schematic of forced swimming test (FST) with restraint stress; FST were recorded before restraint stress and two-day interval after restraint stress. B Comparing the immobility with the control mice before and after restrain stress, Tmem108 mutant (Tmem108 −/−) mice exhibited mania-like behavior in the tests (Tmem108 + / + mice n = 22, Tmem108 −/− mice n = 18). C Tmem108 mutant mice had serious mortality in the restraint stress. D Schematic of seizure inducement. Scopolamine was injected 30 min before applying pilocarpine to minimize the peripheral side effects. E Representative time courses of seizure development by repeated pilocarpine injection. Mice of two genotypes were subjected to pilocarpine injection every 30 min and scored for the seizure stage. F An increased number of pilocarpine injections were needed to reach stage 5 seizure for Tmem108 mutant mice (Tmem108 + / + mice n = 7, Tmem108 −/− mice n = 8). (Unpaired T-test analysis; * p < 0.05, *** p < 0.001)