Fig. 2

Selection of LV-Rufy3-shRNA and dose selection of LV-Rufy3-shRNA, LV-Rufy3, and 8p-CPT under normal conditions. a Representative bands of Rufy3 expression using three types of LV-Rufy3-shRNAs under the normal condition. LV-Rufy3-shRNA2 downregulated Rufy3 expression. b Representative bands of Rufy3 expression using three doses of LV-Rufy3-shRNAs (12, 18, and 24 µl/kg) under normal conditions. c Representative bands of Rufy3 expression using three doses of LV-Rufy3 (10, 15, and 20 µl/kg) under normal conditions. f Representative bands of Rap1 expression using three doses of 8p-CPT (2, 3, and 4 mg/kg) under normal conditions. d, e, g Quantitative analysis of Rufy3 and Rap1 expressions in different groups. The normal group was used as the standard. Data are shown as mean ± SEM (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. LV-NC or Vehicle group; ^#P < 0.05, ^##P < 0.01, vs. 12 µl/kg-LV-shRNA group or 10 µl/kg-LV-Rufy3