Fig. 8
Effect of LV-shRNA and LV-Rufy3 on cortical cell apoptosis and degradation, brain edema, and neurological score after SAH. a Double immunofluorescence analysis of TUNEL staining (red, Alexa Fluor 555) and neuronal marker (NeuN; green, Alexa Fluor 488) was performed to assess neuronal apoptosis at 24 h after SAH. b Fluoro-Jade C staining (green) was performed to evaluate neuronal degeneration and arrows pointed to FJC-positive cells. c Quantitative analysis of apoptotic neuron percentage. d Quantitative analysis of Fluoro-Jade C positive cells/mm2 in brain sections in each group. e Double immunofluorescence of MBP (green, Alexa Fluor 488) and neuronal marker (NeuN; red, Alexa Fluor 555), and Rufy3 mainly located in the neurons. f Brain water content. g Neurological scoring. Scale bars = 100 μm. ***P < 0.001 vs. Sham group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LV-NC1 groups; &P < 0.05, &&&P < 0.001 vs. LV-NC2 group; $P < 0.05, $$$P < 0.001 vs. LV-Rufy3 group