Fig. 2

DHA treatment reduces the HI induced neuronal loss. a NeuN immunofluorescence in adult (P42) cortex and hippocampus (coronal). Green: NeuN, blue: DAPI. Red arrows indicate the NeuN⁺ cell loss in cortex (layer I–VI are labeled). Images in the boxes at the right top corner of NeuN chenel: magnified images of each boxed area. White arrows in the magnified images indicate the NeuN⁺ cell loss in CA3 areas of hippocampus. Rectangles in the images of merged chenel indicate the ROI for quantification (cortex: 602.5 μm * 605 μm * 6 per rat; CA1: 500 μm * 185 μm * 6 per rat; CA3: 310 μm * 720 μm * 6 per rat). Scale bars = 1 mm (200 μm for the magnified images). b–d Quantitation of NeuN⁺ cell numbers in the rectangles of panel a. Relative cell number index = NeuN⁺ cell numbers/average NeuN⁺ cell numbers of sham group%