Fig. 2

Neuronal loss, astrocyte activation, and formation of neurofibrillary tangles in the hippocampal formation of the 11-months-old 6xTg mouse model of AD. a–c Representative coronal sections of the dorsal hippocampus, entorhinal cortex, and ventral hippocampus from 11-months-old WT and 6xTg mice stained with antibodies for NeuN, GFAP, and phosphorylated tau (P-Tau). d–f In the 6xTg mice, the number of GFAP positive cells and p-Tau(AT-8) positive cells were significantly increased compared with WT mice, but the number of NeuN positive cells was significantly decreased in comparison with WT mice in the dorsal hippocampus, entorhinal cortex, and ventral hippocampus. All data are given as means ± SEM (n = 3 for WT, 3 for 6xTg; One-way ANOVA, *p < 0.05,**p < 0.01, ***p < 0,001, ****p < 0.0001). Scale bar, 50 µm