Fig. 7

CDK5 deficiency reduces long-range projections from the DS to the M2, thalamus, and BLA. A Experimental procedure. On the 14th day after LV/Cas9-sgRNA injection, AAV-hSyn-mCherry (red) was unilaterally injected into the DS. B Scheme of the simplified DS projection pathways. The DS pathway has been simplified, and only the representative DS projection targets are displayed. M2, secondary motor cortex; Tha: thalamic nucleus; BLA, basolateral amygdaloid nucleus. C Coronal section and analysis graph showing anterograde labeling 30 days after AAV injection in the DS (scale bar = 100 μm); the enlarged image shows multiple mCherry-labeled neurons in the DS (scale bar = 10 μm). D–F M2 (D), thalamus (E), and BLA (F) areas are labeled with mCherry projected from the DS. Data are represented as the mean ± SEM, n = 3 sections from three mice per group. One-way ANOVA, Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 vs WT