Fig. 1
From: Genome-wide translation control analysis of developing human neurons
Human neuronal differentiation model and experimental design. A H9 human embryonic stem cell line was differentiated into neuroprogenitor cells, which were induced to differentiate into neurons by FGF2 removal for 30 days. Each developmental stage was tested for correspondent markers. hESC (green: TRA1-60; Red: OCT4), NPC (Red: NESTIN) and Neuron (white: MAP2). Blue: DAPI. Scale bars = 100 µm. B Experimental design. Samples from NPCs in different points of differentiation were collected for Ribosome Profiling and RNA sequencing analysis. C Transcriptomic analysis of differentiating NPCs. RNAseq libraries prepared in duplicates from ED and Neurons were compared with NPC libraries. Differentially expressed genes are indicated according to FC > 2, FDR < 0.01. Blue: upregulated; Red: downregulated. D DAVID and IPA Gene Ontology analysis of RNAseq differentially expressed transcripts in ED and Neurons (FDR < 0.01). E Differentiation markers analysis. Plot with Riboseq and RNAseq fold-change values (normalized to NPC) of neuroepithelial, neural differentiation, and canonical neuronal markers