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Fig. 3 | Molecular Brain

Fig. 3

From: Genome-wide translation control analysis of developing human neurons

Fig. 3

Translational Control of synaptic genes. A Immunofluorescence showing positive presynaptic and post-synaptic markers in 30 days Neurons (green: HOMER1; Red: SYN1; white: MAP2. Blue: DAPI. Scale bars = 100 µm. B Classification of the differential expressed synaptic genes between NPC and neuron accord with their mode of regulation. C SynGO enrichment analysis and total Unique terms quantification of the translation-regulated genes in Neurons in relationship to NPCs. Categories with significant enrichment (FDR < 0.01) in SynGO processes are shown. Dark blue bars: Translational induced category; Light blue bars: up-regulated (TE constant) category. D RNAseq and Riboseq fold-change plots of synaptic vesicles genes Up-regulated and translationally regulated in Neurons compared to NPC. Corresponding TE heatmap of v-ATPase complex subunits, selected RNA Up, TE Down genes, and selected translationally induced genes at each differentiation time-point are indicated. Data are presented as log2 of TE value at each time point. E Experimental design used to confirm translation regulation of selected genes. F ATP6V0D1, NAPG, and RAB3A mRNA are strongly associated with heavy polysomes in neurons. The graphic represents an average of 3 independent experiments. **** indicates p < 0.0001. G VAMP2 is more induced at the protein level than at mRNA level during the differentiation of NPCs into neurons. Comparative protein levels were determined by western blot meanwhile mRNA levels were determined by qPCR. The analysis was performed in 4 independent biological samples

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