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Fig. 1 | Molecular Brain

Fig. 1

From: mTORC1 function in hippocampal parvalbumin interneurons: regulation of firing and long-term potentiation of intrinsic excitability but not long-term contextual fear memory and context discrimination

Fig. 1

Homozygous conditional knock-out of Rptor in PV interneurons causes a deficit in mTORC1 activity. A Left, representative images of EYFP-positive PV interneurons (white arrows) in PV-Raptor-WT, PV-Raptor-Het and PV-Raptor-Homo mice injected with AAV2/9-DIO-EYFP in dorsal CA1 hippocampus. Right, representative images of Raptor-positive (red) EYFP-expressing PV interneurons (white arrows, co-labeling) in PV-Raptor-WT, PV-Raptor-Het and PV-Raptor-Homo mice. Scale bar 100 µm. B Summary graph showing reduced percentage of EYFP-positive cells that are also Raptor-positive in PV-Raptor-Homo mice relative to PV-Raptor-Het and PV-Raptor-WT mice (n = 7 sections from 3 PV-Raptor-WT mice, 6 sections from 3 PV-Raptor-Het mice, and 7 sections from 3 PV-Raptor-Homo mice, from 3 independent experiments). C Representative images showing EYFP-positive cells (green), S6S240/244 phosphorylation (red) and co-labeling (merged) in CA1 hippocampus of PV-Raptor-WT, PV-Raptor-Het and PV-Raptor-Homo mice. D Summary graph showing reduced p-S6 immunofluorescence in CA1 PV interneurons of PV-Raptor-Homo mice relative to PV-Raptor-Het and PV-Raptor-WT mice (n = 5 mice/group from 5 independent experiments, scale bar 50 µm). **** p < 0.0001, * p < 0.05, ns not significant

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Molecular Brain

ISSN: 1756-6606