Fig. 3
Conditional knock-out of Rptor in PV interneurons impairs LTPIE. A Representative traces (top) and time plot for all cells (bottom) of depolarization evoked firing showing long-lasting increase of firing in the group receiving HFS (filled circles, HFS) but not in the non-tetanized control group (open circles, No HFS) in CA1 PV interneuron from PV-Raptor-WT mice. B-D Summary plots of spikes number (B), latency to first spike (C) and first spike threshold (D) measured at -5 to 0 min baseline versus 10–15 min (left), or 25–30 min (right) post-HFS in cells of the tetanized group (HFS, top) and control group (No HFS, bottom). Individual data points before and after are joined by lines; means ± sem are indicated to the side for each group. E–H Similar data representation showing absence of long-lasting potentiation of intrinsic excitability at 10–15 min and 25–30 min after HFS in PV interneurons from PV-Raptor-Het mice. I–L Similar data representation showing a block of long-lasting potentiation of intrinsic excitability at 10–15 min, but not at 25–30 min, after HFS in PV interneurons from PV-Raptor-Homo mice. M and N Summary plots of spike increases relative to baseline for all cells measured at 10–15 min (M) and 25–30 min (N) after HFS in PV interneurons, showing block of LTPIE at 10–15 and 25–30 min after HFS in PV-Raptor-Het mice, and at 10–15 min post HFS in PV-Raptor-Homo mice, relative to PV-Raptor-WT mice. *** p < 0.001, ** p < 0.01, * p < 0.05, ns not significant