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Fig. 1 | Molecular Brain

Fig. 1

From: Increased spine PIP3 is sequestered from dendritic shafts

Fig. 1

Experimental procedure to observe PIP3 dynamics with FLIMPA3 in hippocampal organotypic slices. A Principle of the use of FLIMPA3 to observe PIP3 dynamics. PIP3 production induces a conformational change in FLIMPA3 through the binding of the PH domain to PIP3, leading to an increase in FRET between monomeric GFP (mGFP) and sREARCh. B FLIMPA3 was expressed in CA1 pyramidal neuronal cells in hippocampal organotypic slices of rat brains. Then, fluorescence lifetime imaging was performed on dendritic spines and primary and secondary dendritic shafts by two-photon microscopy. DG indicates dentate gyrus

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Molecular Brain

ISSN: 1756-6606