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Fig. 2 | Molecular Brain

Fig. 2

From: High glucose and palmitic acid induces neuronal senescence by NRSF/REST elevation and the subsequent mTOR-related autophagy suppression

Fig. 2

The induction of HGP activated the mTOR signal pathway and inhibited the autophagy function (a), (b) Western blotting image of p-mTOR, mTOR and p62 in PC12 cells at control and HGP. c Immunofluorescence staining of ATG7 in PC12 cells at control and HGP. d Immunofluorescence staining of p62 in PCNs. Scale bar, 25 μm. e, f Detection of autophagy flux in PC12 cells under the treatment of BafA1(50 nmol,4 h). Data are mean ± SD. n = 3 independent experiments for (a), (b), (d), (e) and (f), n = 4 independent experiments for (c). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Unpaired t test for (a), (b) and (c), two-way ANOVA for (e) and (f)

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Molecular Brain

ISSN: 1756-6606