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Fig. 1 | Molecular Brain

Fig. 1

From: The cyclin G-associated kinase (GAK) inhibitor SGC-GAK-1 inhibits neurite outgrowth and synapse formation

Fig. 1

Effects of GAK inhibition on neurodevelopment in mice. A Outline of the procedure. The ROI is shown within the yellow dotted curves, in which synaptic puncta were quantified (Right). B Cell imaging of neurons treated with 10 μM SGC-GAK-1 or dimethysulfoxide (DMSO) as the control. C Total neurite length per number of neurons in samples treated with 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, and 40 μM SGC-GAK-1 or DMSO (N = 6, each concentration). D Cell imaging of neurons treated with DMSO and stained with anti-synaptophysin 1 and anti-SHANK2 antibodies. E Cell imaging of neurons treated with 10 μM SGC-GAK-1 and stained with anti-synaptophysin 1 and anti-SHANK2 antibodies. F Total number of synapses per number of neurons in samples treated with SGC-GAK-1 (same concentrations as in C) or DMSO (N = 6, each concentration). G Cell imaging of neurons treated with DMSO and stained with SMI-31 or NF-200. H Cell imaging of neurons treated with 2.5 μM SGC-GAK-1 and stained with SMI-31 or NF-200. I SMI-31 staining: Neurite intensity per number of neurons in samples treated with SGC-GAK-1 (same concentrations as in C) or DMSO. J NF200 staining: Neurite intensity per number of neurons in samples treated with SGC-GAK-1 (same concentrations as in C) or DMSO (N = 3, each concentration). K Cell imaging of neurons treated with 2.5 μM SGC-GAK-1, 2.5 μM erlotinib, or DMSO and stained with MAP2. L SGC-GAK-1 treatment: Neurite intensity per number of neurons (stained with MAP2) in samples treated with SGC-GAK-1 (same concentrations as in C and N = 3, each concentration) or DMSO (N = 6). M Erlotinib treatment: Neurite intensity per number of neurons (stained with MAP2) in samples treated erlotinib (N = 3, each concentration, which is the same as in C) or DMSO (N = 6). Image size: 899.04 µm × 899.04 µm (B, G, H, K); 455.44 µm × 455.44 µm (D, E). Total neurite length/number of neurons, total number of synapses/number of neurons, and total neurite intensity/number of neurons were significant (*) at p < 0.05/8 after Bonferroni’s correction and the error bars indicate standard deviation

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