Fig. 2

Increased gene transduction efficiency of AAV2-retro YF mutation in cortico-cortical projections. A A schematic of the expression efficiency assay in the cortico-cortical projection. The original or mutant virus was injected into the S1 cortex of the mouse brain. B Representative images of the injection and the contralateral sides in AAV2-retro-CAG-EGFP (top) and AAV2-retroYF-CAG-EGFP (bottom). EGFP signals were counted at the contralateral sides. The white arrows indicate EGFP labeled neurons in the contralateral sides (right). C The histogram of the fluorescent intensity of EGFP-positive neurons (n = 4 mice [original], n = 4 mice [mutant], p < 0.001, U = 123,049, Wilcoxon's rank sum test). D A schematic of the retrograde transfer efficiency assay. The original or mutant virus was mixed with Red Retrobeads and injected into the S1 cortex of the mouse brain. E Representative images showing EGFP signals (green) and retrobeads (magenta) in the contralateral S1 regions. The white arrows indicate EGFP- and retrobeads-double positive neuron. F The mean ratio of the number of retrobeads- and EGFP- positive neurons out of all retrobeads-positive neurons is shown (mean ± sem; n = 7 mice [original], n = 6 mice [mutant], p = 0.303, Welch's two-tailed t-test, t = 1.093, df = 8.789). All scale bars, 100 μm