Fig. 1

Expression of genes involved in the synthesis of Ub and Ub-like proteins and the genes encoding E1 and E2 enzymes. For this report, the data shown were extracted from datasets derived by genome-wide transcriptome sequencing (RNA-Seq) [13]. The original database is freely available on the GEO repository (www.ncbi.nlm.nih.gov/geo/) with the accession number GSE154677. The analysis covers the temporal pattern of expression of the major genes in the Ub system. RNA was extracted from the cerebral cortex of mice in four stages: embryonic Days 11 (E11), 13 (E13), and 17 (E17) and postnatal Day 1 (PN1). RNA abundance is reported in transcripts per million (TPM), and only genes with TPM values ≥ 2 were considered to be actively transcribed, following the criteria presented in [25]. Figure shows the expression of genes involved in the synthesis of Ub (A), Ub-like proteins (B), and genes encoding Ub-activating E1 enzymes (C) and Ub-conjugating E2 enzymes (D, E)