Fig. 1

Fgf11 knockdown in the ARC decreases body weight and fat mass, and improves glucose metabolism in HFD-fed mice. Adult male C57/BL6 mice were fed HFD for 14 days after bilateral injection of lentivirus expressing shFgf11 and GFP into the ARC. A Coronal section of ARC. GFP indicates lentiviral infection. B mRNA expression of Fgf11 in micro-dissected ARC sample following injection of shFgf11-expressing lentivirus. C Body weight and weight gain of NCD-fed mice during the experimental period. D Food intake and average food intake of NCD-fed mice. E Body weight and weight gain of HFD-fed mice during the experimental period. F Food intake and average food intake of HFD-fed mice. G Lean mass and H fat mass. I Glucose tolerance test (GTT) and J insulin tolerance test (ITT) after lentivirus injection. Data are mean ± SEM; two-tailed t-test was used for statistical analysis. *p < 0.05, **p < 0.01 and ***p < 0.001 (non-silencing shRNA control versus shFgf11). n = 5–16 mice/group