Fig. 4
From: Silencing of hypothalamic FGF11 prevents diet-induced obesity
FGF11 regulates Npy gene expression. siRNA-mediated Fgf11 knockdown was conducted in NPY/AgRP co-expressing hypothalamic cells. A–C mRNA expression of A Fgf11, B Npy, and C AgRP following Fgf11 knockdown in N41 cells. D N41 cells were transfected with empty vector (E/V) or HA-tagged FGF11 and Fgf11 overexpression and knockdown were confirmed by immunoblotting. E Npy mRNA expression after Fgf11 knockdown with Fgf11 overexpression (O/E). N41 cells were exposed to 0 mM glucose (glc) medium with Fgf11 knockdown, and mRNA expression of F Fgf11 and G Npy was measured. N41 cells were treated with 2DG with Fgf11 knockdown and mRNA expression of H Fgf11 and I Npy was evaluated. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, n = 6–11