Fig. 6
From: Silencing of hypothalamic FGF11 prevents diet-induced obesity
FGF11 regulates Npy gene expression via GSK3-dependent CREB regulation in NPY/AgRP co-expressing hypothalamic cells. N41 cells were treated with dimethyl sulfoxide (vehicle) or BIO (1 μM) for 1, 3, or 6 h. A, B mRNA expression of Fgf11 and Npy after the treatment of BIO. C, D Western blots of GSK3, β-catenin, and CREB following the treatment of BIO. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (vehicle versus BIO). n = 4–6. E–H N41 cells were transfected with non-silencing siRNA control or siFgf11 for 48 h, followed by the treatment with vehicle or BIO for 6 h. E, F mRNA expression of Fgf11 and Npy after Fgf11 knockdown with BIO treatment. G, H Western blots of GSK3, β-catenin, and CREB after Fgf11 knockdown with BIO treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, n = 3–9