Fig. 1
Stimulation of protein synthesis in SOM-INs by chemical and optogenetic induction of persistent and transient LTP, respectively. a Protocol of chemical persistent LTP induction (top left), representative images (middle), cumulative distribution plots and summary bar graphs (bottom; each group 3 independent slice experiments from 3 animals, 3–6 sections analyzed per experiment) of puromycin immunofluorescence in SOM-INs of control SOM-EYFP-WT mice (left) and conditional SOM-EYFP-Raptor-KO mice (right), showing increase in fluorescence after DHPG treatment relative to Sham-treatment in control mice, but not in conditional knockout mice. Summary bar graph (mean ± SEM; SOM-EYFP-WT mice, Sham = 395 cells and DHPG = 396 cells; SOM-EYFP-Raptor KO mice, Sham = 457 cells and DHPG = 487 cells). b Protocol of optogenetic (TBSopto) transient LTP induction (top), representative images (middle), cumulative distribution plot and summary bar graph (bottom; each group 4 independent slice experiments from 2 animals, 4–6 sections analyzed per experiment) of puromycin immunofluorescence in SOM-INs of SOM-EYFP-WT mice, showing an increase in fluorescence after TBSopto relative to control (unstimulated) slices. Summary bar graph (mean ± SEM; Control 570 cells and TBSopto 761 cells). c Protocol of consecutive induction of TBSopto (in absence of puromycin) and repeated mGluR1 (in presence of puromycin) LTP (top), representative images (middle), cumulative distribution plot and summary bar graph (bottom; each group 6–8 independent slice experiments from 3–4 animals, 3–6 sections analyzed per experiment) of puromycin immunofluorescence in SOM-INs of SOM-EYFP-WT mice, showing that the increase in fluorescence after DHPG treatment is impaired by prior application of TBSopto. Summary bar graph (mean ± SEM; Ctl, Sham 781 cells and DHPG 805 cells; TBSopto, Sham 925 cells and DHPG: 869 cells). In all panels: arrows indicate cells with colocalization of EYFP with puromycin immunofluorescence; scale bars, 50 µm; Kolmogorov–Smirnov tests (cumulative distribution tests) or Student’s t-tests (group mean tests), * p < 0.05, ** p < 0.01, **** p < 0.0001 and ns not significant