Fig. 3
From: Olfactory marker protein contains a leucine-rich domain in the Ω-loop important for nuclear export
Mutations in the leucine-rich sequence alter the nuclear export efficacy of OMP. a, b Representative pictures of OMP-IR in HEK293T cells expressing a OMPWT or b OMPA. c Quantification of the cell numbers (%) showing nuclear OMP-IR with apparent signal voids surrounding dense nuclear foci among all OMP-IR cells transfected with OMPWT (filled blue circles) and OMPA (filled red squares). n = 5 experiments each. One-tailed Student’s T test: OMPWT vs. OMPA, *P = 7.36 × 10–10. d The OMP-IR ratio of the nucleus/cytoplasm (N/C ratio). n = 100 cells for OMPWT and 102 cells for OMPA. One-tailed Student’s T test: OMPWT vs. OMPA, **P = 6.97 × 10–34. e OMP-IR from cytoplasmic (C) and nuclear (N) fractions extracted from HEK293T cells expressing OMPWT or OMPA. f N/C ratio for the cell-fractional OMP-IR detected by western blotting in e. One-tailed Student’s T test: OMPWT vs. OMPA, #P = 0.0089. g Hydrophobicity of OMPWT (blue) and OMPA (red) around the Ω-loop. +, mutated residues