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Fig. 3 | Molecular Brain

Fig. 3

From: Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H

Fig. 3

Impact of leukodystrophy-associated POLR3B R103H substitution on the time-course of Pol III assembly

FLAG-tagged POLR3B WT or FLAG-tagged POLR3B R103H were transfected in HEK293 in triplicate and harvested at time points indicated in the flow chart (A). Affinity purification was performed as in Fig. 2. Shown in (B) are histogram of statistically significant association with POLR3B WT (n = 3) and POLR3B R103H (n = 3). POLR3B WT associated proteins were deemed statistically significant when they had a False discovery rate (FDR) of p < 0.05. C) The model summarizes POLR3B multi-step subcomplex formation following interaction between POLR3B and 10 other Pol III subunits. The #-marked subunit was used as bait. The @-marked subunit was statistically significant at 16 h and onward and the &-marked subunit was only statistically significant at 16 h

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