Fig. 2

Both D₁R and D₂R mediate Ca²⁺ response in adult mPFC astrocytes. A Representative traces of DA (50 μM)-induced Ca²⁺ before and after treatment of D₁R blocker (20 μM SCH-23390), D₂R (2 μM haloperidol), and neuronal activity blocker (0.5 μM TTX) in mPFC astrocytes. B Summary bar graph of Ca²⁺ peak ratio in adult mPFC astrocytes without drugs (Ctrl, n = 13 ROIs from 3 slices) or after treatment of SCH-23390 (n = 18 ROIs from 6 slices), haloperidol (n = 11 ROIs from 5 slices), and TTX (n = 9 ROIs from 2 slices). C Representative traces DA-induced Ca²⁺ in mPFC astrocytes of MAO-B WT and KO mice. D Summary bar graph of Ca²⁺ peak ratio in MAO-B WT (n = 23 ROIs from 5 slices) and MAO-B KO (n = 34 ROIs from 8 slices) mPFC astrocytes. E A working model of the age-dependent switch of DA-induced astrocyte Ca²⁺ signaling. Means ± SEM. Student’s t-test, ***p < 0.001; ****p < 0.0001