Fig. 7
Computer simulation of nRT neuron firing. a Representative electrical membrane potential of the virtual soma containing wild-type (WT) Cav3.2 channels in response to a 200 ms-long hyperpolarizing current injection of − 0.65 nA (left panel) and corresponding number of spikes during the rebound as a function of the current injected (right panel). b–g Legend same as a but for TN-associated Cav3.2 variants. The dashed line depicts the number of spikes for WT channels for comparison. h Minimum current injection (rheobase) necessary to trigger rebound action potentials: WT (− 0.585 nA), S187L (− 0.568 nA), A802V (− 0.606 nA), E819K (− 0.561 nA), P1120L (− 0.528 nA), E2280H (− 0.471 nA), and E2291K (− 0.559 nA). i Rebound firing frequency at -0.65 nA current injection: WT (15 Hz), S187L (35 Hz), A802V (10 Hz), E819K (45 Hz), P1120L (55 Hz), E2280H (55 Hz), and E2291K (20 Hz). J–p Legend same as a–g but for depolarizing current injections. Representative membrane potentials are shown in response to 0.2 nA current injection. q Minimum current injection (rheobase) necessary to trigger tonic action potentials: WT (0.0257 nA), S187L (0.0258 nA), A802V (0.0260 nA), E819K (0.0255 nA), P1120L (0.0251 nA), E2280H (0.0254 nA), and E2291K (0.0255 nA). r Action potential frequency at 0.2 nA current injection: WT (170 Hz), S187L (180 Hz), A802V (170 Hz), E819K (180 Hz), P1120L (185 Hz), E2280H (175 Hz), and E2291K (175 Hz)