Fig. 3

Pre-weaning enrichment does not affect the ontogeny of novel object recognition. A–F Open field (OF) locomotor activity analysis and G–J novel object recognition (NOR) memory test was performed using video tracking in SH and EE mice grouped by age: A, B, E, G, H P19–P21 and C, D, F, I, J P25–P27. A, C Total distance in the peripheral sector and the center of the arena during OF (B, D) Total time the mice are mobile during the OF test for each age group. E, F Total distance and average speed of the SH and EE mice during the OF test for each age group. G, I Time spent exploring both familiar (f) and novel (n) objects for each age group during the NOR test. In both SH and EE mice age P25–P27, mice spent more time exploring novel object compared to the familiar. H, J Discrimination index (DI) calculated as time spent exploring novel object divided by total object exploration time. DI values are non-significantly different between SH and EE mice in both age groups. A preference for novel object is seen in older juvenile (P25–P27) as demonstrated by one-sample t-test above chance (0.5) (#) p = 0.01 and (\(\bot\)) p = 0.0027. All other statistics were done using a two-sample t-test (**p < 0.01). For each bar, the number of animals used for the quantification is reported; individual dots represent the mean per animal