Fig. 1

Immature dentate gyrus phenotype assessed using typical molecular markers in Cn mutant mice. a–h Immunostaining images and quantified bar graphs of calbindin (a; t = 11.20, df = 22, P < 0.0001), GluA1 (b; t = 16.66, df = 22, P < 0.0001), GluA2 (c; t = 10.00, df = 4, P = 0.0006), doublecortin (d; left graph: t = 4.39, df = 4, P = 0.012; right graph: t = 11.66, df = 826, P < 0.0001), PSA-NCAM (e; F test: P < 0.0001; Mann Whitney test: P < 0.0001), calretinin (f; t = 11.23, df = 22, P < 0.0001), phospho-CREB (g; F test: P < 0.0001; Mann Whitney test: P = 0.0091), and CREB (h; t = 0.24, df = 10, P = 0.81). i Western blot images and quantified bar graphs of phospho-CREB and CREB. Graphs show relative levels of phospho-CREB over total CREB. P = 0.0085. The original blots are included in Additional file 1: Fig. S3. j Expression levels of Dsp, tdo2, and Drd1a mRNA (Dsp: t = 6.95, df = 13, P < 0.0001; Tdo2: t = 10.64, df = 14, P < 0.0001; Drd1a: t = 2.71, df = 13, P = 0.018). Bar graphs show means ± SEM. Each dot represents one mouse, except for the right graph in d. The positions of doublecortin-positive cells are shown as relative values between the subgranular layer (0) and the border with the molecular layer (1) (d, right graph; each dot represents one cell). Scale bars: a upper panels 500 μm, lower panels 50 μm; b, c left panels 500 μm, right panels 100 μm; d, e left panels 300 μm, right panels 50 μm; f left panels 200 μm, right panels 50 μm; g, h 50 μm. Relative intensity means the ratio of the immunoreactive intensity obtained from the mutants to that of the controls. *P < 0.05, **P < 0.01, Students t-test. Con, control mice; Mut, Cn mutant mice