Fig. 2
From: Proteins secreted by brain arteriolar smooth muscle cells are instructive for neural development
The secretome of mouse brain VSMCs promotes neuron development. a–d Representative images of hippocampal neurons immunostained with an anti-Tuj1 antibody (green) and stained with phalloidin-647 (red) after culture for 12 (a), 36 (b), 48 (c), and 72 (d) hours. e Quantification of neurons entering the consolidation phase after culture for 12 h. f Fold changes in neurons in which all neurites were in a specialized form between the two groups with or without denatured VSMC-CM. g Cumulative curve of the percentages of neurons with different neurite numbers after treatment with MBVSMC-CM at 12, 36, 48, and 72 h after plating. h–j Quantification of average total neurite length per neuron after cultured for 36 (h), 48 (i), 72 h (j)