Table 1 PCR conditions and sequence information for the primers
Target genes | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Temp (°C) | Cycles |
|---|---|---|---|---|
TNF-α | CATCTTCTCAAAATTCGAGTGACAA | ACTTGGGCAGATTGACCTCAG | 60 | 25 |
iNOS | CCCTTCCGAAGTTTCTGGCAGCAGC | GGCTGTCAGAGCCTCGTGGCTTTGG | 70 | 30 |
IL-1β | CTTTGAAGAAGAGCCCATCC | TTTGTCGTTGCTTGGTTCTC | Realtime | 40 |
IL-6 | CCAATTTCCAATGCTCTCCT | ACCACAGTGAGGAATGTCCA | Realtime | 40 |
Cyclo | TGGAGAGCACCAAGACAGACA | TGCCGGAGTCGACAATGAT | Realtime | 40 |
GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 60 | 25 |