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Fig. 1 | Molecular Brain

Fig. 1

From: 14-3-3γ haploinsufficiency leads to altered dopamine pathway and Parkinson’s disease-like motor incoordination in mice

Fig. 1

Ywhag knockout heterozygote mice identified with reduced expression of 14-3-3γ. a–c Representative western blotting image (a) and quantitative graph (b,c) show reduced expression of 14-3-3γ in the striatum (ST) and substantia nigra (SN) homogenates of the 14-3-3γ HET mice brain as compared to the CTL mice (n = 3 per group). d Immunohistochemistry results show that the 14-3-3γ immunostaining intensity in the ST and SN regions is mainly expressed in neurons indicated as NeuN ( +) cells, and a decrease in the 14-3-3γ intensity was detected in 14-3-3γ HET mice compared with CTL mice (scale bar: 0.2 μM). e, f Quantitative graphs of 14-3-3γ immunostaining intensity in NeuN ( +) cells in the ST (e) and the SN (f) regions of the 14-3-3γ HET mice brain and the CTL mice brain (n = 3 per group). Results are presented as means ± SEM; *P < 0.05, **P < 0.01 and ***P < 0.001. (CTL littermate wild-type control, HET heterozygous, NeuN neuronal nuclei, DAPI 4,6-diamidino-2-phenylindole, SEM standard error of mean)

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