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Fig. 2 | Molecular Brain

Fig. 2

From: 14-3-3γ haploinsufficiency leads to altered dopamine pathway and Parkinson’s disease-like motor incoordination in mice

Fig. 2

Decreased expression of phosphorylated tyrosine hydroxylase and insignificant change in expression of the tyrosine hydroxylase in the 14-3-3γ heterozygous mice brain. a–c Representative western blotting images (a) and quantitative graphs (b, c) of the phosphorylated tyrosine hydroxylase (pTH)/the tyrosine hydroxylase (TH) ratio (b) and total TH expression (c) in whole brain homogenates of the 14-3-3γ HET mice and CTL mice (n = 3 per group). e-i Representative western blotting images (e) and quantitative graphs (f-i) of pTH/TH ratio (f,g) and TH expression (h,i) in the striatum (ST) and the substantia nigra (SN) homogenates of the 14-3-3γ HET mice brain and CTL mice brain (n = 3 per group). j Immunohistochemistry results show reduced expression of pTH but not TH in the ST and the SN regions of the 14-3-3γ HET mice brain and CTL mice brain (scale bar, 0.2 μM). k, l Quantitative graphs of TH immunostaining intensity in the ST (k) and the SN (l) regions of the 14-3-3γ HET mice brain and the CTL mice brain (n = 4 per group). m, n Quantitative graphs of pTH immunostaining intensity normalized by TH in the ST (m) and the SN (n) regions of the 14-3-3γ HET mice brain and CTL mice brain (n = 4 per group). Results are presented as means ± SEM; *P < 0.05, **P < 0.01 and ***P < 0.001. (CTL littermate wild-type control, HET heterozygous, pTH phosphorylated tyrosine hydroxylase, TH tyrosine hydroxylase, ST striatum, SN substantia nigra, NeuN neuronal nuclei, DAPI 4,6-diamidino-2-phenylindole, SEM standard error of mean, ns non-significant)

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