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Fig. 3 | Molecular Brain

Fig. 3

From: 14-3-3γ haploinsufficiency leads to altered dopamine pathway and Parkinson’s disease-like motor incoordination in mice

Fig. 3

Decreased dopamine level and dopamine transporter expression in the 14-3-3γ heterozygous mice brain. a Schematic diagram of dopamine assay using competitive ELISA assay. b–d Results of dopamine levels in the whole brain (b), the striatum (ST) (c) and substantia nigra (SN) (d) homogenates of the 14-3-3γ HET mice brain and CTL mice brain (n = 3 per group). e, f Representative western blotting image (e) and quantitative graph (f) of the dopamine transporter (DAT) expression in brain homogenates of the 14-3-3γ HET mice and CTL mice (n = 4 per group). g Immunohistochemistry results showing reduced expression of DAT intensity in the ST and SN regions of the 14-3-3γ HET mice brain and CTL mice brain (scale bar, 0.2 μM). h, i Quantitative graphs of DAT immunostaining intensity in the ST (h) and SN (i) regions of the 14-3-3γ HET mice brain and CTL mice brain (n = 4 per group). Results are presented as means ± SEM; *P < 0.05, **P < 0.01 and ***P < 0.001. (CTL littermate wild-type control, HET heterozygous, pTH phosphorylated tyrosine hydroxylase, TH tyrosine hydroxylase, DAT dopamine transporter, ST striatum, SN substantia nigra, NeuN neuronal nuclei, DAPI 4,6-diamidino-2-phenylindole, SEM standard error of mean, ns non-significant)

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