Fig. 1
Synaptic tagging was induced in the ACC by tagging TBS in middle-aged male mice. Tagging was recorded from a slice of a male mouse by extracellular field potential recording. a Microscopy photograph showing the relative location of ACC slice and MED64 probe and the arrangement of the microelectrodes (electrode size 50 × 50 μm, the interpolar distance of electrodes 150 μm). Schematic diagram of stimulation site (S1: blue circle for the strong TBS; S2: red circle for the weak TBS) of microelectrodes in the ACC. Spatial distribution of extracellular field potential induced by strong TBS on channel 26 (marked as a blue circle) in layers II and weak TBS on channel 46 (marked as a red circle) in layers V of the ACC. b Schematic diagram of the recording procedure for sites S1 and S2. c The Markov Random Field model was introduced to smooth and visualize the low-resolution 8 × 8 MED64 data. The graph model consists of the observation at site i (Oi), the state at site i (Si), and the state at a neighboring site j (Sj). d As a generative process, the value of Oi is generated from Si, while the value of Si also depends on its 4-connection neighborhood denoted as Sj. The original 8 × 8 MED64 record for a single time frame. Then, a 256 × 256 projection of the original MED64 data that is both spatially and temporally smoother with a color bar. Finally, the temporal evolving of the 3D surface of the projected 256 × 256 MED64 recording. e The temporal evolving of the 3D surface on the site S1 in the middle-aged mice. f Summarized plot of the time-varying fEPSP slope in all activated channels for site S1 from 1 slice of a middle-aged male mouse. g The temporal evolving of the 3D surface on site S2 of the middle-aged mice. h Summarized plot of time-varying fEPSP slopes in all activated channels for tagging TBS protocol from the 1 slice